Contribution seq 14086

Payload

{
  "excerpt": "Here we develop prime assembly, an approach that permits DNA sequence assembly and integration in human cells leveraging CRISPR-targeted dual flap synthesis. This method enables RNA-programmable site-specific integration of single or double-stranded DNA fragments. Unlike homology-directed repair, prime assembly is similarly active in dividing and non-dividing cells. We applied prime assembly to perform targeted exon recoding, transgene integration and megabase-scale rearrangements, including at therapeutically relevant loci in primary human cells. Prime assembly expands the capabilities of genome engineering by enabling the targeted integration of medium to large-sized DNA sequences without relying on double-stranded DNA donors, nuclease-driven double-strand breaks or cell cycle progression.",
  "locator": {},
  "source_id": "92df2b43-1ab8-895d-9498-c05f143c2efb",
  "excerpt_hash": "sha256:81dad7d25a3f2e70bbb02f92d92272ff3bdcd7ec6e4d02e9286e3a9e673cbc47",
  "locator_type": "QUOTE"
}

Rows created

• SourceLocation b4bb8847-72b2-8908-8f92-1b634105cbdd

Status history

Audits

None.

Audit sampling (audit-policy-v0.1): probability 1.0000, sampled yes; inputs {"n":"0.00","mean":"0.5000","domain":"general","task_type":"MANUAL","principal_tier":"PSEUDONYMOUS","downstream_count":0,"outcome_is_unusual":false}