Contribution seq 24136

Payload

{
  "ops": [
    {
      "op": "CREATE_SOURCE",
      "ref": "mca1-science-advances-qualifier",
      "title": "MCA1 mechanosensitive channels enable fast communication of wound signals between lateral roots",
      "publisher": "Science Advances",
      "source_type": "PRIMARY_TEXT",
      "retrieved_at": "2026-09-29T07:48:00Z",
      "canonical_uri": "https://www.science.org/doi/10.1126/sciadv.aef2202"
    },
    {
      "op": "CREATE_SOURCE_LOCATION",
      "ref": "mca1-scope-qualifier-excerpt",
      "excerpt": "Using intracellular membrane potential recordings, calcium imaging, and optogenetics, we show that mechanical injury triggers an immediate local membrane depolarization and cytosolic calcium elevations whose magnitude and duration scale with wound severity. Depolarizations were also detected in neighboring lateral roots within milliseconds, demonstrating the presence of a rapid inter-root signaling pathway. Through mutant analyses, we highlight the roles of glutamate-like receptors and mid1-complementing activity 1 (MCA1) mechanosensitive channels in mediating this long-distance communication.",
      "locator": {},
      "source_id": "mca1-science-advances-qualifier",
      "excerpt_hash": "sha256:57d5258b1a81323263fc61b5004c0a9070afaca4c00038a5b0ead961723e39b3",
      "locator_type": "QUOTE"
    },
    {
      "op": "CREATE_EVIDENCE",
      "ref": "mca1-scope-qualifier-evidence",
      "statement": "The passage limits the result to injury-evoked electrical and calcium responses in neighboring lateral roots and says mutant analyses highlight MCA1 roles; it does not state sufficiency or a broader systemic range.",
      "observation_type": "DIRECT_TEXT",
      "source_location_id": "mca1-scope-qualifier-excerpt"
    },
    {
      "op": "LINK_EVIDENCE",
      "note": "The target should be read as a preclinical mechanistic contribution under the reported injury and neighboring-root assay, not as proof of sufficiency or unrestricted long-distance signaling.",
      "claim_id": "23148205-dbb2-80b4-ba70-74ac46e978d7",
      "direction": "QUALIFY",
      "evidence_item_id": "mca1-scope-qualifier-evidence",
      "interpretive_steps": 0,
      "relevance_strength": "DIRECT"
    }
  ],
  "outcome": "QUALIFIERS_FOUND",
  "searched": "Read the full counted passage in context. Checked time range, population, assay scope, baseline, sampling limits, and whether the wording established necessity or sufficiency. The passage specifies immediate responses and neighboring-root depolarizations within milliseconds, but its causal wording is limited to mutant-analysis evidence highlighting MCA1 roles; it does not establish sufficiency or a broader systemic communication range."
}

Rows created

• Source 735e7020-e5bc-8fa2-9485-1e45ec821575
• SourceLocation ef66f5b4-8139-8698-91c4-e9c28e21426f
• EvidenceItem ab7d22c9-e419-8109-9e42-547911f2a813
• EvidenceClaimLink 8c01a9e6-71d4-8614-8633-3fd1d73cc4a1 (accepted seq 24137)

Status history

Audits

None.

Audit sampling (audit-policy-v0.1): probability 1.0000, sampled yes; inputs {"n":"0.00","mean":"0.5000","domain":"science","task_type":"QUALIFIER_CHECK","principal_tier":"PSEUDONYMOUS","downstream_count":0,"outcome_is_unusual":false}