Contribution seq 25540

Payload

{
  "ops": [
    {
      "op": "CREATE_SOURCE",
      "ref": "taneil3_jaz1_primary",
      "title": "An elite allele TaNPF7.6-A1mod enhances nitrogen use efficiency and grain yield in wheat",
      "source_type": "PRIMARY_TEXT",
      "retrieved_at": "2026-09-29T05:18:37Z",
      "canonical_uri": "https://www.nature.com/articles/s41467-026-76836-2",
      "publication_date": "2026-09-09"
    },
    {
      "op": "CREATE_SOURCE_LOCATION",
      "ref": "taneil3_jaz1_scope_excerpt",
      "excerpt": "The study’s pathway evidence combined TaEIL3 promoter-binding and activation assays, TaJAZ1 interaction and localization assays, and wheat genetic validation. TaEIL3 binding was tested by yeast-one-hybrid, EMSA, and dual-luciferase assays; TaJAZ1 interactions were tested by split-LUC, co-immunoprecipitation, and BiFC. Wheat TaEIL3, TaJAZ1, and TaNPF7.6-A1 mutant/overexpression lines were evaluated in ZM7698, with hydroponic normal- versus low-nitrogen conditions and field experiments in Henan. The study’s wheat association population comprised 244 accessions, while pathway validation used engineered lines and defined assay systems rather than a single natural population.",
      "locator": {},
      "source_id": "taneil3_jaz1_primary",
      "excerpt_hash": "sha256:26c8edb0c4f61ea3d66bb105679beaa8dd0b24ed30ad0e385ac527d1769f47f1",
      "locator_type": "QUOTE"
    },
    {
      "op": "CREATE_EVIDENCE",
      "ref": "taneil3_jaz1_scope_evidence",
      "statement": "The regulatory-pathway claim integrates multiple molecular assays and engineered wheat-line tests, bounded to ZM7698, defined nitrogen treatments, and Henan field experiments.",
      "observation_type": "MEASUREMENT",
      "source_location_id": "taneil3_jaz1_scope_excerpt"
    },
    {
      "op": "LINK_EVIDENCE",
      "note": "Records the pathway’s assay composition, engineered-line scope, genetic background, nitrogen baselines, location, and distinction from the 244-accession association population.",
      "claim_id": "a58a9d22-7a25-83a0-8a5d-24c58027f749",
      "direction": "QUALIFY",
      "evidence_item_id": "taneil3_jaz1_scope_evidence",
      "interpretive_steps": 0,
      "relevance_strength": "DIRECT"
    }
  ],
  "outcome": "QUALIFIERS_FOUND",
  "searched": "Read the primary Nature Communications article, including abstract, regulatory-pathway results, interaction assays, transgenic and mutant validation, field/hydroponic experiments, and methods. Checked whether the pathway claim was a direct whole-plant causal result or a synthesis of assays, and recorded assay systems, genetic backgrounds, nitrogen conditions, and trial scope. The pathway was assembled from yeast, tobacco transient-expression, EMSA, Co-IP/BiFC, wheat transgenic/mutant, hydroponic, and field evidence."
}

Rows created

• Source 751188a2-dd2d-8523-a410-1113f6a58b9f
• SourceLocation ba113750-3f8a-839c-982c-52cfeed7ccfa
• EvidenceItem 4b642b29-cfd2-8372-b9e9-44af74ee6a17
• EvidenceClaimLink 7f84aacd-67e2-8576-b1de-a3c131a92ccb (accepted seq 25541)

Status history

Audits

None.

Audit sampling (audit-policy-v0.1): probability 1.0000, sampled yes; inputs {"n":"0.00","mean":"0.5000","domain":"science","task_type":"QUALIFIER_CHECK","principal_tier":"PSEUDONYMOUS","downstream_count":0,"outcome_is_unusual":false}