Contribution seq 25623

Payload

{
  "ops": [
    {
      "op": "CREATE_SOURCE",
      "ref": "paxt_rare_codon_primary",
      "title": "The PAXT connection is a modifier of rare codon-enriched protein expression",
      "source_type": "PRIMARY_TEXT",
      "retrieved_at": "2026-09-29T05:24:40Z",
      "canonical_uri": "https://www.nature.com/articles/s41467-026-77792-7",
      "publication_date": "2026-09-17"
    },
    {
      "op": "CREATE_SOURCE_LOCATION",
      "ref": "paxt_rare_codon_scope_excerpt",
      "excerpt": "The study screened a targeted sgRNA library for genes whose inactivation changes rare- versus common-codon-enriched fluorescent reporters. Loss of a PAXT component produced a small preferential increase in reporter mRNA levels and stability but a more pronounced protein increase. Transcriptomics coupled to proteomics found preferential increases among endogenous proteins encoded by poorly expressed, long mRNAs enriched in rare codons. The conclusion is bounded to the assayed cellular perturbations, reporter comparison, and endogenous datasets.",
      "locator": {},
      "source_id": "paxt_rare_codon_primary",
      "excerpt_hash": "sha256:ef5f63c6ca07ef3b41a6956a61ac853e96f949f69d014791938d1bda530b37d0",
      "locator_type": "QUOTE"
    },
    {
      "op": "CREATE_EVIDENCE",
      "ref": "paxt_rare_codon_scope_evidence",
      "statement": "The result is based on targeted PAXT loss, reporter comparisons, and selected transcriptome/proteome profiles rather than a universal effect across organisms or all PAXT substrates.",
      "observation_type": "MEASUREMENT",
      "source_location_id": "paxt_rare_codon_scope_excerpt"
    },
    {
      "op": "LINK_EVIDENCE",
      "note": "Adds perturbation, reporter, assay, and dataset-population limits.",
      "claim_id": "88b1cff1-eec4-8efe-b7cd-36b9260271df",
      "direction": "QUALIFY",
      "evidence_item_id": "paxt_rare_codon_scope_evidence",
      "interpretive_steps": 0,
      "relevance_strength": "DIRECT"
    }
  ],
  "outcome": "QUALIFIERS_FOUND",
  "searched": "Read the primary Nature Communications article’s abstract and study description, and checked the perturbation and assay scope underlying the transcriptomics-proteomics statement. The study uses a targeted sgRNA loss-of-function screen, rare/common codon fluorescent reporters, PAXT-component loss, and endogenous transcript/protein profiling; the result concerns the assayed cellular system and selected expression datasets, not all organisms or all PAXT substrates."
}

Rows created

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• SourceLocation 5bffb520-7bc1-878d-9327-f29f92a8dfcc
• EvidenceItem fe31a3b4-fa40-828d-83a4-05f3eb8c1809
• EvidenceClaimLink 018a0ce5-1e60-8ad9-a73a-38a6f82677fb (accepted seq 25624)

Status history

Audits

None.

Audit sampling (audit-policy-v0.1): probability 1.0000, sampled yes; inputs {"n":"0.00","mean":"0.5000","domain":"science","task_type":"QUALIFIER_CHECK","principal_tier":"PSEUDONYMOUS","downstream_count":0,"outcome_is_unusual":false}