Contribution seq 25636

Payload

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      "ref": "synthetic_promoter_primary_support",
      "title": "A modular synthetic promoter toolkit for programmable gene control in activated and chronically stimulated T cells",
      "publisher": "Nature Communications",
      "source_type": "PRIMARY_TEXT",
      "retrieved_at": "2026-09-29T05:25:00Z",
      "canonical_uri": "https://www.nature.com/articles/s41467-026-77730-7",
      "publication_date": "2026-09-29"
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      "ref": "synthetic_promoter_support_excerpt",
      "excerpt": "We quantitatively profile promoter performance in HEK293 and Jurkat cells, evaluate them for orthogonality, and integrate them into Boolean logic gate architectures and network topologies to minimize basal transcriptional leakiness and improve ON/OFF ratios. We test selected synthetic promoters in primary human CD8⁺ T cells, under endogenous TF regulation, and we further characterize the NR4A2-responsive promoter subset in vitro and in tumor-infiltrating engineered T cells in a melanoma xenograft model, demonstrating dynamic and reversible activation.",
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      "statement": "The primary text directly reports the stated cell-line profiling and selected-promoter experiments in primary CD8-positive T cells, in vitro, and melanoma xenograft tumor-infiltrating engineered T cells.",
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      "note": "Directly supports the experimental-system scope.",
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      "relevance_strength": "DIRECT"
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  "outcome": "FOUND",
  "searched": "Reviewed the Nature Communications primary-text passage describing promoter profiling and the selected-promoter experiments, including cell lines, primary human CD8-positive T cells, in-vitro NR4A2 testing, and the melanoma xenograft model."
}

Rows created

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• SourceLocation cf5e98c3-3255-8334-9170-a8b1ccf77c13
• EvidenceItem 69e92374-0798-8225-83b7-73e9dca3a32d
• EvidenceClaimLink 1200b514-7e51-8033-8bda-17ef28674885 (accepted seq 25637)

Status history

Audits

None.

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