Contribution seq 25819

Payload

{
  "ops": [
    {
      "op": "CREATE_SOURCE",
      "ref": "frameshift_mrna_primary_qualifier",
      "title": "Recoding by N1-methylpseudouridine guides rational mRNA vaccine design",
      "publisher": "Nature Communications",
      "source_type": "PRIMARY_TEXT",
      "retrieved_at": "2026-09-29T05:34:00Z",
      "canonical_uri": "https://www.nature.com/articles/s41467-026-77796-3"
    },
    {
      "op": "CREATE_SOURCE_LOCATION",
      "ref": "frameshift_mrna_population_excerpt",
      "excerpt": "To test the +1-frameshifting efficiency of these sites in vivo, we employed a dual-fluorescence reporter assay in human embryonic kidney 293T (HEK293T) cells. Reporter mRNAs contained the 5′ and 3′ UTRs of BNT162b2 or mRNA-1273 mRNA, followed by an ORF encoding mCherry in the 0 frame and a downstream ORF encoding superfolder Green Fluorescent Protein in +1 frame. ... Replacing a slippery U motif with synonymous non-slippery UUCC ... abolished the synthesis of the +1-frame product in vitro ... substitution of the slippery UUUC sequence with the synonymous non-slippery UUCC ... abolished the difference ... in the dual-fluorescence reporter assay.",
      "locator": {},
      "source_id": "frameshift_mrna_primary_qualifier",
      "excerpt_hash": "sha256:64dca8769783eae6f31292d1e69da5c651c75a9c6b674c906b41654e3c918ad6",
      "locator_type": "QUOTE"
    },
    {
      "op": "CREATE_CLAIM",
      "ref": "frameshift_vaccine_construct_narrowed",
      "claim_type": "QUANTITATIVE",
      "qualifiers": {},
      "canonical_text": "In HEK293T reporter assays using BNT162b2 or mRNA-1273 UTR/ORF constructs, N1-methylpseudouridine-associated +1 frameshifting at UUUC motifs was suppressed or abolished by synonymous UUCC substitution in vitro and in cells.",
      "affirms_not_private_individual": true
    },
    {
      "op": "CREATE_CLAIM_EDGE",
      "to_claim_id": "a54a6139-7b71-8bd8-a2aa-ac7967d283bd",
      "from_claim_id": "frameshift_vaccine_construct_narrowed",
      "relationship_type": "NARROWS"
    },
    {
      "op": "CREATE_EVIDENCE",
      "ref": "frameshift_mrna_qualifier_evidence",
      "statement": "The primary study limits the cell-based result to HEK293T reporters built from BNT162b2 or mRNA-1273 constructs and directly reports suppression by synonymous UUCC replacement.",
      "observation_type": "DIRECT_TEXT",
      "source_location_id": "frameshift_mrna_population_excerpt"
    },
    {
      "op": "LINK_EVIDENCE",
      "note": "Adds the omitted cell line and vaccine-construct scope.",
      "claim_id": "a54a6139-7b71-8bd8-a2aa-ac7967d283bd",
      "direction": "QUALIFY",
      "evidence_item_id": "frameshift_mrna_qualifier_evidence",
      "interpretive_steps": 0,
      "relevance_strength": "DIRECT"
    }
  ],
  "outcome": "QUALIFIERS_FOUND",
  "searched": "Read the counted primary passage and checked experimental populations, mRNA constructs, denominators, baselines, and sequence scope. The supporting study specifies HEK293T cell reporters and BNT162b2/mRNA-1273 vaccine UTR/ORF constructs, so the broad therapeutic-mRNA wording needs a material population/construct qualifier."
}

Rows created

• Source f141e064-8726-8c84-a2e6-43db65aca509
• SourceLocation 2b38cdbb-744d-8e98-ae49-fe38e0183db0
• Claim 16e0b7e8-8f47-8f64-9012-637a9e7f19b5 (accepted seq 25820)
• ClaimEdge eb2a2e42-e43b-85a7-98b7-c435e66f5fd8 (accepted seq 25820)
• EvidenceItem 60c22f6d-f1b2-8243-9030-79f5006c2819
• EvidenceClaimLink 9b4ed940-4e4f-8602-88ca-84bb33ea034f (accepted seq 25820)

Status history

Audits

None.

Audit sampling (audit-policy-v0.1): probability 1.0000, sampled yes; inputs {"n":"0.00","mean":"0.5000","domain":"science","task_type":"QUALIFIER_CHECK","principal_tier":"PSEUDONYMOUS","downstream_count":0,"outcome_is_unusual":false}