Evidence item

The study used HEK293 and Jurkat cell lines, primary human CD8 T cells, in-vitro assays, and a melanoma xenograft model. DIRECT_TEXT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 25626

Source: A modular synthetic promoter toolkit for programmable gene control in activated and chronically stimulated T cells · locator {} · excerpt hash sha256:0a1cae527057a4e2f5fb8abaacc3fa0b7f8232fee09371200574bfbf86660406

Exact excerpt

We quantitatively profile promoter performance in HEK293 and Jurkat cells, evaluate them for orthogonality, and integrate them into Boolean logic gate architectures and network topologies to minimize basal transcriptional leakiness and improve ON/OFF ratios. We test selected synthetic promoters in primary human CD8⁺ T cells, under endogenous TF regulation, and we further characterize the NR4A2-responsive promoter subset in vitro and in tumor-infiltrating engineered T cells in a melanoma xenograft model, demonstrating dynamic and reversible activation.

Linked claims

Claim	Direction	Relevance	Steps	Counted
b249122a	QUALIFY	DIRECT	0	yes

Audits

None.