Evidence item

The study reports modification-associated +1 frameshifting at UUUC motifs and suppression by synonymous codon replacement. MEASUREMENT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 17966

Source: Recoding by N1-methylpseudouridine guides rational mRNA vaccine design · locator {} · excerpt hash sha256:f8879e0fb75da1d27629fe1004a92d629b3481d7e7b4a87e7e4f869394deab63

Using mRNA-based dual-fluorescence reporters in cells, fully in vitro reconstituted translation system, and single-molecule FRET microscopy, we show that m1Ψ increases +1 frameshifting on UUUC motifs in therapeutic mRNAs. ... Replacing slippery UUUC motifs with UUCC or UUUU eliminates this effect.