Evidence item

In human cells, synonymous substitution of the BNT162b2 slippery UUU C sequence with UUC C abolished the difference between unmodified and m1Ψ-modified reporters. MEASUREMENT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 25829

Source: Recoding by N1-methylpseudouridine guides rational mRNA vaccine design · locator {} · excerpt hash sha256:460a140fdc2f69614cb00bd9e117d3a0cda961199c093e83c8ade19932f5a1de

This suppression was also evident in human cells: substitution of the slippery UUU C sequence with the synonymous non-slippery UUC C abolished the difference between the unmodified and m1Ψ-modified mRNA of BNT162b2 ... in the dual-fluorescence reporter assay.