Evidence item

The regulatory-pathway claim integrates multiple molecular assays and engineered wheat-line tests, bounded to ZM7698, defined nitrogen treatments, and Henan field experiments. MEASUREMENT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 25540

Source: An elite allele TaNPF7.6-A1mod enhances nitrogen use efficiency and grain yield in wheat · locator {} · excerpt hash sha256:26c8edb0c4f61ea3d66bb105679beaa8dd0b24ed30ad0e385ac527d1769f47f1

Exact excerpt

The study’s pathway evidence combined TaEIL3 promoter-binding and activation assays, TaJAZ1 interaction and localization assays, and wheat genetic validation. TaEIL3 binding was tested by yeast-one-hybrid, EMSA, and dual-luciferase assays; TaJAZ1 interactions were tested by split-LUC, co-immunoprecipitation, and BiFC. Wheat TaEIL3, TaJAZ1, and TaNPF7.6-A1 mutant/overexpression lines were evaluated in ZM7698, with hydroponic normal- versus low-nitrogen conditions and field experiments in Henan. The study’s wheat association population comprised 244 accessions, while pathway validation used engineered lines and defined assay systems rather than a single natural population.

Linked claims

Claim	Direction	Relevance	Steps	Counted
8027f749	QUALIFY	DIRECT	0	yes

Audits

None.