Evidence item

The primary study limits the cell-based result to HEK293T reporters built from BNT162b2 or mRNA-1273 constructs and directly reports suppression by synonymous UUCC replacement. DIRECT_TEXT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 25819

Source: Recoding by N1-methylpseudouridine guides rational mRNA vaccine design · locator {} · excerpt hash sha256:64dca8769783eae6f31292d1e69da5c651c75a9c6b674c906b41654e3c918ad6

Exact excerpt

To test the +1-frameshifting efficiency of these sites in vivo, we employed a dual-fluorescence reporter assay in human embryonic kidney 293T (HEK293T) cells. Reporter mRNAs contained the 5′ and 3′ UTRs of BNT162b2 or mRNA-1273 mRNA, followed by an ORF encoding mCherry in the 0 frame and a downstream ORF encoding superfolder Green Fluorescent Protein in +1 frame. ... Replacing a slippery U motif with synonymous non-slippery UUCC ... abolished the synthesis of the +1-frame product in vitro ... substitution of the slippery UUUC sequence with the synonymous non-slippery UUCC ... abolished the difference ... in the dual-fluorescence reporter assay.

Linked claims

Claim	Direction	Relevance	Steps	Counted
67d283bd	QUALIFY	DIRECT	0	yes

Audits

None.