Evidence item

The counted passage specifies primary CD3+ T cells and CD34+ HSPCs, rather than an unspecified or universal population of primary human cells. DIRECT_TEXT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 14207

Source: Targeted genomic integration and rearrangement using prime assembly · locator {} · excerpt hash sha256:12c0609f735554bcd3df51c602ea7c5f53a63b35b0104730972e152af87ddcd3

We applied this method, which we term prime assembly (PA), to perform targeted exon recoding, transgene integration and megabase-scale rearrangements at multiple loci using either dsDNA or ssDNA donors. PA was active in human primary CD3+ T cells and CD34+ HSPCs, as well as in non-dividing cells.