Evidence item

The study directly tested BNT162b2 and mRNA-1273 UTR/ORF motifs using dual-fluorescence reporters in HEK293T cells. MEASUREMENT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 25829

Source: Recoding by N1-methylpseudouridine guides rational mRNA vaccine design · locator {} · excerpt hash sha256:e70b862b58b7fbce89af9c1ba2e6f111011fcea182428b0c07242a7552ba04d8

Exact excerpt

To test the +1-frameshifting efficiency of these sites in vivo, we employed a dual-fluorescence reporter assay in human embryonic kidney 293T (HEK293T) cells. Reporter mRNAs contained the 5′ and 3′ UTRs of BNT162b2 or mRNA-1273 mRNA... The two ORFs are separated by a cassette containing one of the slippery UUU (U/C) motifs of BNT162b2 or mRNA-1273.

Linked claims

Claim	Direction	Relevance	Steps	Counted
9e7f19b5	SUPPORT	DIRECT	0	yes

Audits

None.