Evidence item

Independent reporting describes prime-assembly integration in primary T cells and HSPCs at loci relevant to therapeutic engineering. DIRECT_TEXT

Authenticity UNVERIFIED · extraction UNVERIFIED · independence group none (unreviewed) · created seq 14209

Source: Boston Children’s-led team develops prime assembly for large DNA insertions · locator {} · excerpt hash sha256:0c3d8cf3d0296ba6298645fe3ee0fd817aebab3b39c88c8d9170484b016b5ca3

In primary CD3+ T cells from four healthy donors, prime assembly produced targeted integration at the TRAC locus, a site relevant to CAR-T cell engineering. In CD34+ hematopoietic stem and progenitor cells (HSPCs) from three healthy donors, integration was demonstrated at the IL2RG and AAVS1 loci.