Claim

In HEK293T reporter assays using BNT162b2 or mRNA-1273 UTR/ORF constructs, N1-methylpseudouridine-associated +1 frameshifting at UUUC motifs was suppressed or abolished by synonymous UUCC substitution in vitro and in cells.

Type QUANTITATIVE · truth-evaluable: yes · status at snapshot: ACTIVE · created seq 25819

29 words; atomic claims are usually under 25a conjunction joins clauses that may be separately evaluable

Referenced 95 times: checked 0, looked up by assistants 0, viewed 95, shared 0. A count of attention, not evidence.

Topics

Not filed under any topic yet.

Assessment

Supported

Score: 0.9975 under ledger-default@0.3.0 at snapshot 27745

3 of 4 review checks done · 1 counted · 2 by the author only

3 independent evidence lineages · review checks 1 of 4 · stability HIGH

Main unresolved issue: 3 counted evidence items have no independence review; repeats of one origin may be counted as several coverage:16e0b7e8-8f47-8f64-9012-637a9e7f19b5

Not yet independently audited.Evidence reviewed so far supports this claim, but only 1 of 4 review checks has been done.

Related: NARROWS outgoing: Supported

Why?

Strongest counted support: In human cells, synonymous substitution of the BNT162b2 slippery UUU C sequence with UUC C abolished the difference between unmodified and m1Ψ-modified reporters. weight 2.000000 evidence

Review gaps: qualifiers reviewed, opposing search done, independence reviewed

What would most change this: One independent, direct measurement that contradicts this claim would move it from Supported to Supported.

Hide calculation

0.9975 under ledger-default@0.3.0 at snapshot 27745 · state Supported · Review checks: 1 of 4 · stability HIGH · 3 sources, 3 independent lineages

Model ledger-default@0.3.0 (the default model, not the answer) · snapshot 27745 · trace sha256:eae6c2e7226e03433a6d91c5f62b809fbed9572fde22f4e850e2300214b2abe7

Last changed at entry seq 25830; nothing logged since has borne on this claim.

How the number was reached

Each counted link is weighed by multiplying the factors the model declares; a factor it does not declare is not shown. Every factor name below links to what that number means.

Link	Working	Weight	Counted as
059a619f support	relevance DIRECT 2.0 × observation MEASUREMENT 1.0 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	2.000000	2.000000
b8fe5806 support	relevance DIRECT 2.0 × observation MEASUREMENT 1.0 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	2.000000	2.000000
a3b23777 support	relevance DIRECT 2.0 × observation MEASUREMENT 1.0 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	2.000000	2.000000

prior 0.50 → log-odds 0.000000 + 6.000000 (the counted weights) = 6.000000 → probability 0.9975

Under neighbouring settings of the same model: 0.9852 to 0.9996, a spread of 0.0144.

Half-even rounding, six places for weights and four for the probability. The same inputs under the same model reproduce this exactly; that is what the trace hash above is for. How a score is worked out explains every factor, why it is there, and what the model sets it to.

Your view

Registered views

Nobody has registered a view yet.

Registered views are personal belief, not evidence: they never change Galedra's assessment. Affiliations are self-declared and shown only for groups of 5 or more.

Evidence for

The primary study reports m1Ψ-associated +1 frameshifting on UUUC motifs and elimination of the effect after replacement with UUCC or UUUU.

From Recoding by N1-methylpseudouridine guides rational mRNA vaccine design Read 2026-09-29 Relevance DIRECT Steps 0 Counted
The study directly tested BNT162b2 and mRNA-1273 UTR/ORF motifs using dual-fluorescence reporters in HEK293T cells.

From Recoding by N1-methylpseudouridine guides rational mRNA vaccine design Read 2026-09-29 Relevance DIRECT Steps 0 Counted
In human cells, synonymous substitution of the BNT162b2 slippery UUU C sequence with UUC C abolished the difference between unmodified and m1Ψ-modified reporters.

From Recoding by N1-methylpseudouridine guides rational mRNA vaccine design Read 2026-09-29 Relevance DIRECT Steps 0 Counted

Evidence against

None.

Qualifications

None.

Suppressed as dependent

None.

Inferences

Concluded from

None.

Used as a premise in

None.

An inference is interpretation, not evidence: it never changes the assessment of its conclusion. Each premise is a claim with its own evidence.

Threads

None. Threads are for disagreements about how a determination was made — a statement whose figures are not in the passage it rests on, two sources that may share an origin. Something about the world belongs in the evidence above.

Claim edges

From	Relationship	To
9e7f19b5	NARROWS	67d283bd

Review checklist

Review checks: 1 of 4

2 checks by this claim's own author, alongside the independent review above. 1 OPPOSING EVIDENCE SEARCH · 1 QUALIFIER CHECK. A check by the person who recorded a claim does not raise its review coverage.

Check	Done	By
qualifiers reviewed	no
opposing search done	no
independence reviewed	no
primary source reviewed	yes	059a619f, b8fe5806, a3b23777

Summary

Supported. The main support is: In human cells, synonymous substitution of the BNT162b2 slippery UUU C sequence with UUC C abolished the difference between unmodified and m1Ψ-modified reporters. 85824c23

A search for evidence in the opposing direction found something; see the counted evidence. ff8bce46

Review checks: 1 of 4. coverage:16e0b7e8-8f47-8f64-9012-637a9e7f19b5

Generated by stub-v0.1; every sentence cites graph ids.

Contribution history

seq	event	detail
25819	APPENDED
25820	ACCEPT	AUTOMATIC_AFTER_VALIDATION
25829	TASK_RESULT	by 1efd24fe (accepted)

Score trace

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  "links": [
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      "group": null,
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  ],
  "model": "ledger-default@0.3.0",
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  "variants": {
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    "permissive": "0.9996",
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  },
  "code_hash": "sha256:c61a6fdf35a754a0d7e958df149036a083c0eaeb7d2d2e52704ec32b0c36def7",
  "contested": false,
  "stability": "HIGH",
  "claim_type": "QUANTITATIVE",
  "config_hash": "sha256:86768038080b8ab5233f1e4b7aba10af05b5bb44caa432095150b90e81209809",
  "probability": "0.9975",
  "provisional": true,
  "evidence_sum": "6.000000",
  "snapshot_seq": 25830,
  "prior_log_odds": "0.000000",
  "support_groups": 3,
  "model_dependent": false,
  "review_coverage": "0.25",
  "truth_evaluable": true,
  "assessment_state": "SUPPORTED",
  "review_checklist": {
    "qualifiers_reviewed": {
      "by": [],
      "ok": false
    },
    "opposing_search_done": {
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      "ok": false
    },
    "independence_reviewed": {
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      "ok": false
    },
    "primary_source_reviewed": {
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      ],
      "ok": true
    }
  },
  "contradict_groups": 0,
  "rounding_boundary": false,
  "posterior_log_odds": "6.000000",
  "not_applicable_reason": null,
  "independence_unreviewed": 3
}