Claim

Prime assembly enables RNA-programmable, site-specific integration of medium-to-large DNA sequences in human cells without relying on nuclease-driven double-strand breaks or cell-cycle progression.

Type OBSERVATIONAL · truth-evaluable: yes · status at snapshot: ACTIVE · created seq 14090

Referenced 135 times: checked 3, looked up by assistants 0, viewed 76, shared 56. A count of attention, not evidence.

Topics

Biology · 1efd24fe Medicine · 1efd24fe

Assessment

Supported

Score: 0.9985 under ledger-default@0.3.0 at snapshot 27745

3 of 4 review checks done · 1 counted · 2 by the author only

4 independent evidence lineages · review checks 1 of 4 · stability HIGH

Main unresolved issue: 4 counted evidence items have no independence review; repeats of one origin may be counted as several coverage:ce749ecc-4d0c-8512-a6c1-35580a685248

Not yet independently audited.Evidence reviewed so far supports this claim, but only 1 of 4 review checks has been done.A quoted passage from genengnews.com and pubt.io was not found on the page when Galedra fetched it.

Related: NARROWS incoming: Supported

Why?

Strongest counted support: GEN quotes the paper as describing RNA-programmable site-specific integration active in dividing and non-dividing cells. weight 1.800000 evidence

Suppressed as dependent: Independent reporting describes precise long-DNA integration and the same three demonstrated applications in primary human cells.

Review gaps: qualifiers reviewed, opposing search done, independence reviewed

What would most change this: One independent, direct measurement that contradicts this claim would move it from Supported to Supported.

Hide calculation

0.9985 under ledger-default@0.3.0 at snapshot 27745 · state Supported · Review checks: 1 of 4 · stability HIGH · 6 sources, 4 independent lineages

Model ledger-default@0.3.0 (the default model, not the answer) · snapshot 27745 · trace sha256:d7d5b4cfb5a0cbd0fe68e5107a60a6c76167e43132ca1147d58cfb2f8e39e289

Last changed at entry seq 23706; nothing logged since has borne on this claim.

How the number was reached

Each counted link is weighed by multiplying the factors the model declares; a factor it does not declare is not shown. Every factor name below links to what that number means.

Link	Working	Weight	Counted as
03160e6c support	relevance STRONG 1.2 × observation DIRECT_TEXT 0.9 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	1.080000	1.080000
7f5f5700 support	relevance DIRECT 2.0 × observation DIRECT_TEXT 0.9 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	1.800000	1.800000
1d08514a support	relevance DIRECT 2.0 × observation DIRECT_TEXT 0.9 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	1.800000	1.800000
767fd4e9 qualify	relevance DIRECT 2.0 × observation DIRECT_TEXT 0.9 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	1.800000	0.000000 (non directional)
1588453c support	relevance STRONG 1.2 × observation DIRECT_TEXT 0.9 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	1.080000	0.000000 (dependent strongest only, kept e92be119)
e92be119 support	relevance DIRECT 2.0 × observation DIRECT_TEXT 0.9 × interpretation 0 steps, 1 − 0.15 × 0 1.000000 × authenticity UNVERIFIED 1.0 × extraction UNVERIFIED 1.0 × provenance INDEPENDENT 1.0 × edition NAMED_OR_UNRELATED 1	1.800000	1.800000

prior 0.50 → log-odds 0.000000 + 6.480000 (the counted weights) = 6.480000 → probability 0.9985

Under neighbouring settings of the same model: 0.9894 to 0.9998, a spread of 0.0104.

Half-even rounding, six places for weights and four for the probability. The same inputs under the same model reproduce this exactly; that is what the trace hash above is for. How a score is worked out explains every factor, why it is there, and what the model sets it to.

Your view

Registered views

Nobody has registered a view yet.

Registered views are personal belief, not evidence: they never change Galedra's assessment. Affiliations are self-declared and shown only for groups of 5 or more.

Checked as part of

Statements this claim was broken out of. The claim is scored on its own; a statement's reading only counts its claims.

“Prime assembly enables RNA-programmable, site-specific integration of medium-to-large DNA sequences in human cells without relying on nuclease-driven...” · 1 claim · 29 September 2026
“Prime assembly enables RNA-programmable, site-specific integration of medium-to-large DNA sequences in human cells without relying on nuclease-driven...” · 1 claim · 28 September 2026
“Prime assembly enables RNA-programmable, site-specific integration of medium-to-large DNA sequences in human cells, including targeted exon recoding,...” · 2 claims · 28 September 2026

Evidence for

Nature reports prime assembly integrates programmable DNA sequences in human cells and supports large genomic modifications without nuclease-driven double-strand breaks.

From Targeted genomic integration and rearrangement using prime assembly Read 2026-09-28 Relevance DIRECT Steps 0 Counted
Independent reporting describes precise long-DNA integration and the same three demonstrated applications in primary human cells.

From Prime Assembly Expands Genome Editing with Precise, Large-Scale DNA Integration Read 2026-09-28 Relevance STRONG Steps 0 Counted
Institutional reporting says prime assembly targets long DNA fragments without double-strand breaks or donors and works in nondividing cells.

From Researchers reveal new genomic editing method for correcting multiple mutations at once Read 2026-09-28 Relevance STRONG Steps 0 Counted

Independent institutional account adds support beyond the Nature paper and GenEngNews summary.
The excerpt directly states RNA-programmable site-specific integration in human cells without nuclease-driven double-strand breaks or cell-cycle progression.

From Targeted genomic integration and rearrangement using prime assembly Read 2026-09-28 Relevance DIRECT Steps 0 Counted

The passage explicitly states each component of the claim.
GEN quotes the paper as describing RNA-programmable site-specific integration active in dividing and non-dividing cells.

From Prime Assembly Expands Genome Editing with Precise, Large-Scale DNA Integration Read 2026-09-29 Relevance DIRECT Steps 0 Counted

Evidence against

None.

Qualifications

The source specifies primary human cells and named genome-engineering applications, bounding the claim’s demonstrated scope.

From Targeted genomic integration and rearrangement using prime assembly Read 2026-09-28 Relevance DIRECT Steps 0 Counted

The evidence supports the method claim but materially limits it to reported primary-cell demonstrations and named applications.

Suppressed as dependent

Evidence	Group	Kept instead
d5323712	711e1eea	e92be119

Inferences

Concluded from

None.

Used as a premise in

None.

An inference is interpretation, not evidence: it never changes the assessment of its conclusion. Each premise is a claim with its own evidence.

Threads

None. Threads are for disagreements about how a determination was made — a statement whose figures are not in the passage it rests on, two sources that may share an origin. Something about the world belongs in the evidence above.

Claim edges

From	Relationship	To
6727bbd9	NARROWS	0a685248

Review checklist

Review checks: 1 of 4

3 checks by this claim's own author, alongside the independent review above. 1 OPPOSING EVIDENCE SEARCH · 1 QUALIFIER CHECK · 1 EVIDENCE VERIFICATION. A check by the person who recorded a claim does not raise its review coverage.

Check	Done	By
qualifiers reviewed	no
opposing search done	no
independence reviewed	no
primary source reviewed	yes	1d08514a, 767fd4e9, e92be119

Summary

Supported. The main support is: Institutional reporting says prime assembly targets long DNA fragments without double-strand breaks or donors and works in nondividing cells. dc21983b

1 further item repeats the same origin and is not independent evidence. 711e1eea

Qualifier: The source specifies primary human cells and named genome-engineering applications, bounding the claim’s demonstrated scope. f5f2ff4c

A narrower version of this claim is supported. 6727bbd9

A search for evidence in the opposing direction found none. cf02f192

Review checks: 1 of 4. coverage:ce749ecc-4d0c-8512-a6c1-35580a685248

Generated by stub-v0.1; every sentence cites graph ids.

Contribution history

seq	event	detail
14090	APPENDED
14091	ACCEPT	AUTOMATIC_AFTER_VALIDATION
14102	LINK_EVIDENCE	by 1efd24fe (accepted)
14106	LINK_EVIDENCE	by 1efd24fe (accepted)
14123	LINK_EVIDENCE	by 1efd24fe (accepted)
14126	TASK_RESULT	by 1efd24fe (accepted)
14139	TASK_RESULT	by 1efd24fe (accepted)
23705	LINK_EVIDENCE	by 1efd24fe (accepted)

Score trace

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  "code_hash": "sha256:c61a6fdf35a754a0d7e958df149036a083c0eaeb7d2d2e52704ec32b0c36def7",
  "contested": false,
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