Claim

Model ledger-default@0.1.0 ledger-strict@0.1.0 ledger-default@0.2.0 ledger-strict@0.2.0 ledger-default@0.3.0 (default) ledger-strict@0.3.0 Snapshot seq

Topics

Biology · 1efd24fe Technology · 1efd24fe

Assessment

Why?

Strongest counted support: The study reports modification-associated +1 frameshifting at UUUC motifs and suppression by synonymous codon replacement. weight 2.000000 evidence

Suppressed as dependent: The packet directly supports the experimental systems, UUUC frameshifting result, and suppression by UUCC or UUUU substitution.

Review gaps: qualifiers reviewed, opposing search done, independence reviewed

What would most change this: One independent, direct measurement that contradicts this claim would move it from Supported to Leans supported.

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Checked as part of

Statements this claim was broken out of. The claim is scored on its own; a statement's reading only counts its claims.

• “In cell-based, reconstituted-translation, and single-molecule experiments, N1-methylpseudouridine increased +1 ribosomal frameshifting at UUUC motifs in...” · 1 claim · 29 September 2026
• “In cell-based, reconstituted-translation, and single-molecule experiments, N1-methylpseudouridine increased +1 ribosomal frameshifting at UUUC motifs in...” · 1 claim · 29 September 2026

Evidence for

• The study reports modification-associated +1 frameshifting at UUUC motifs and suppression by synonymous codon replacement.

  From Recoding by N1-methylpseudouridine guides rational mRNA vaccine design Read 2026-09-29 Relevance DIRECT Steps 0 Counted

• The packet directly supports the experimental systems, UUUC frameshifting result, and suppression by UUCC or UUUU substitution.

  From Recoding by N1-methylpseudouridine guides rational mRNA vaccine design Read 2026-09-29 Relevance DIRECT Steps 0 Counted

• An independent Nature study reports m1Psi-associated +1 frameshifting and shows synonymous targeting of slippery sequences reduces frameshifted products.

  From N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting Read 2026-09-29 Relevance STRONG Steps 0 Counted

  Independent paper corroborates the core frameshifting and sequence-optimization components; it does not independently establish every 2026 mechanistic detail.

Evidence against

None.

Qualifications

• The primary study limits the cell-based result to HEK293T reporters built from BNT162b2 or mRNA-1273 constructs and directly reports suppression by synonymous UUCC replacement.

  From Recoding by N1-methylpseudouridine guides rational mRNA vaccine design Read 2026-09-29 Relevance DIRECT Steps 0 Counted

  Adds the omitted cell line and vaccine-construct scope.

Suppressed as dependent

Evidence	Group	Kept instead
112bfea2		7336d48c

Inferences

Concluded from

None.

Used as a premise in

None.

An inference is interpretation, not evidence: it never changes the assessment of its conclusion. Each premise is a claim with its own evidence.

Threads

None. Threads are for disagreements about how a determination was made — a statement whose figures are not in the passage it rests on, two sources that may share an origin. Something about the world belongs in the evidence above.

Claim edges

From	Relationship	To
9e7f19b5	NARROWS	67d283bd

Review checklist

Review checks: 1 of 4

2 checks by this claim's own author, alongside the independent review above. 1 QUALIFIER CHECK · 1 EVIDENCE VERIFICATION. A check by the person who recorded a claim does not raise its review coverage.

Check	Done	By
qualifiers reviewed	no
opposing search done	no
independence reviewed	no
primary source reviewed	yes	00b5e8eb, ea563e3a, 33ea034f, 7336d48c

Summary

Contribution history

seq	event	detail
17962	APPENDED
17963	ACCEPT	AUTOMATIC_AFTER_VALIDATION
17968	LINK_EVIDENCE	by 1efd24fe (accepted)
17987	TASK_RESULT	by 1efd24fe (accepted)
18014	LINK_EVIDENCE	by 1efd24fe (accepted)
25819	TASK_RESULT	by 1efd24fe (accepted)

Score trace

{
  "claim": "a54a6139-7b71-8bd8-a2aa-ac7967d283bd",
  "links": [
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      "link": "588b54a7-0982-8ce3-abb6-47f700b5e8eb",
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      "direction": "SUPPORT",
      "magnitude": "1.080000",
      "relevance": "STRONG",
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      "audit_confirmed": false,
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    {
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      "group": null,
      "reason": "dependent_strongest_only",
      "edition": "NAMED_OR_UNRELATED",
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    },
    {
      "link": "9b4ed940-4e4f-8602-88ca-84bb33ea034f",
      "group": null,
      "reason": "non_directional",
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      "audit_confirmed": false,
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  ],
  "model": "ledger-default@0.3.0",
  "prior": "0.50",
  "variants": {
    "spread": "0.0859",
    "permissive": "0.9821",
    "conservative": "0.8962"
  },
  "code_hash": "sha256:c61a6fdf35a754a0d7e958df149036a083c0eaeb7d2d2e52704ec32b0c36def7",
  "contested": false,
  "stability": "HIGH",
  "claim_type": "QUANTITATIVE",
  "config_hash": "sha256:86768038080b8ab5233f1e4b7aba10af05b5bb44caa432095150b90e81209809",
  "probability": "0.9561",
  "provisional": true,
  "evidence_sum": "3.080000",
  "snapshot_seq": 25820,
  "prior_log_odds": "0.000000",
  "support_groups": 2,
  "model_dependent": false,
  "review_coverage": "0.25",
  "truth_evaluable": true,
  "assessment_state": "SUPPORTED",
  "review_checklist": {
    "qualifiers_reviewed": {
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      "ok": false
    },
    "opposing_search_done": {
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      "ok": false
    },
    "independence_reviewed": {
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      "ok": false
    },
    "primary_source_reviewed": {
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      ],
      "ok": true
    }
  },
  "contradict_groups": 0,
  "rounding_boundary": false,
  "posterior_log_odds": "3.080000",
  "not_applicable_reason": null,
  "independence_unreviewed": 4
}